Today we were gram staining our plates we had made from our cheese and had made new plates using the 3 quadrant zone. When gram staining, it's pretty much almost vital that you get the times correct or else it will destroy your data and alter your results. They way we gram stained was using this substance called Crystal Violet for 30 seconds. 2nd, we washed it off using distilled water for 5 seconds. 3rd, we put on Gram Iodine for 60 seconds. Then washed again with the distilled water. Mind you that we used the little droppers in the bottles it came with so we did a few drops to cover the bacteria. 5th, we put on the Gram Decolorizer for 1-5 seconds. 6th, washed off with distilled water once more. 7th, put on Safranin for 30 seconds and washed it off, yet again, with distilled water.
I learned a new technique of inoculating bacteria and that's the 3 quadrant zone which we spread the cheese in half of the plate, sterilized the inoculation loop, spread the cheese in a quarter of the plate, sterilized the inoculation loop again, then spread the cheese once more over the last quarter. We then taped up the edges and stored it in a "special fridge".
Lastly, we meet with Mrs. Chapman to talk about our upcoming projects and what we have been doing thus far in the semester. :)
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