Today's Color: Pink

Recap: Yesterday I incubated a total of 24 petri dishes. There was a total of 12 different species of bacteria (and a duplicate for each bacteria). I needed to wait 24 hours for the bacteria to incubate to begin the bioremedation experiments. One experiment (experiment 1) would consist of a control tube (just 2 mL of tetrazoilium and 10 drops of corn oil), and other tubes with the same substances but the unique bacteria added in each tube. Another experiment (experiment 2) would consist of just the 2 mL of tetrazolium, and tetrazolium with the unique species of bacteria. 

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Results from incubation of each bacteria

Bacillus Subtilis that has been streaked and incubated for 24 hours. It looks like thick circles that form some kind of worm-like appearance. 

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Experiment 1 and 2

Bacteria: Salmonella enterica 

There was immediate result when adding Salmonella to the tetrazolium and corn oil. Since there are obvious signs of a pink color, it shows that Salmonella is capable of degrading the oil. 

With experiment 1, I tested 10 tubes and one control tube which was just 2mL of tetrazolium and 10 drops of corn oil. 


The types of bacteria used:

• Bacillus subtilis
• E. coli 
• Serratia marcescens 
• Salmonella enterica
• Proteus vulgaris
• Enterococcus faecalis
• Pseudomonas aeroginosa
• Staphylococcus epidermidis 
• Proteus mirabilis  

For the most part, the results showed many of the species of bacteria to take immediate or gradual, but quick effect of changing into a pinkish to dark pink color. Usually, it takes a day or two to take effect (Enterobacter aerogenes), but many of the bacteria tested were much quicker than that. Experiment 2 also was the same, but I excluded corn oil and left it only with tetrazolium and added the same species of bacteria. All same results except P. aeroginosa, E. faecalis and S. epidermidis showed no effect.

[Bacillus subtilis (gram positive) takes immediate effect on just tetrazolium]