Thursday, February 2, 2012

2/1-2/12

I went around campus and searched for various types of leaves. Among the species I collected, there was a leaf from an Arizona Ash tree, Lantana, Cilantro, and Chared. The stomata count was different for each leaf varying from 10-20 stomata openings on the top. I also looked at the bottom side and that ranged from 20-60 stomata openings. This is just practice for now but I would say that the stomata on the bottom has much more than the top side.

The process of extracting the stomata of the plants were:

1. Paint clear nail polish on the leaf
2. Let it dry
3. Use a corner of a piece of tape to help peel off the nail polish
4. Tape that piece to a single microscope slide
5. Place a cover slip on top to try to flatten the nail polish out
6. Look under 4x to get it aligned
7. Change to 10x to get a better look
8. Change to 40x to see the openings

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