Josh introduced me to a new project, which was to look for a way to isolate bacteria from soil. I researched several experiments, but then I was given a mini-lesson on how to conduct this experiment with the help of Josh and Matt. Several mistakes here and there, but I eventually learned how.
Soil Project Continued (Microbes)
Samples of soil. One is directly from the garden, and another is from a path of soil.
What I needed to keep in mind before using the "hood".
- Make sure to clean any contamination with Windex
- What goes in, it does not come out until it is closed and finished.
- Make sure to wash hands!
- Obtain 2 different samples of soil (path and garden). *Make sure to label!
- Have a test tube racket with six test tubes + cap.
- Have the "hood" clean with Windex and have paper towels ready in case of spills.
- Wait for 5-15 minutes with UV-ray light to clean the "hood" from bacteria.
- Turn "blower" on and light on and get ready.
- Have Super Broth and about 4 LB Nutrient Broth in the "hood".
- Make sure to have all materials in the "hood" and do not take anything out!
- Add soil (2 will be garden soil, 2 will be path soil, and one with garden and another with path. *Use yellow loops (do not touch the loop!).
- Once labeled (test tubes), add 4 (2 garden soil and 2 path) 5 ml of LB Nutrient Broth to 4 test tubes.
- *Make sure to use a pipette and graduated cylinder to take out the Broth. If contaminated (Broth), use new broth and pipette.
- Add 5 ml of Super Broth in each test tube remaining (2).
- Note: when taking out media (opening cap), try not to open completely or else it may become contaminated.
- Take test racket to the incubator and wait for alarm or ask. (Maybe 1 hour).
- Repeat sanitary procedures.
- With test tubes in the hood and 6 petri dishes, use loops to swab samples for each tube and apply to each petri dish. Swab the sample in the petri dish several times.
- Label (turn over) with date, name, and media used.
Results
Example of 1 of the 6 with bacteria and other things growing.
Interesting project, and nice pictures :) Have you been able to make any claims based upon your collected evidence? Also, there is a typo in your second sentence that you should fix...
ReplyDeleteI have not made any claims as of right now because I would still need to look at the bacteria under a microscope. However, a claim that I can think of is that there is always bacteria in soil that come in colonies, and by the results in the petri dish, that helped me see the different types of living organisms that grow in the soil.
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